Since the area intensity is in arbitrary unit, it can also be normalised to the BCA assay measurement, DNA content or any other number chosen. To normalise the intensity of the area underneath the peak to the Ponceau staining, measure the intensity of 3 randomly chosen peaks on the Ponceau image, average the measurements and use that value to normalise the data against. The report will automatically pop up on the side. Go to: Analyse→Gels→Label Peaks to get the report.Īlternatively, use the magic wand tool to highlight the area underneath the peak for each lane. Draw the line at where the peak begins and ends (bend in the line) for each peak. On OS X, older versions of Java 8 (prior to 1.8.045)as well as all versions of Java 7 (including 1.7.080)are extremely slow at displaying images. There are several reasons ImageJ can run slowly on macOS. Use the line tool to draw the lines to eliminate the lane background from the calculations. ImageJ becomes very slow after running for a while. It was developed at the University of Wisconsin-Madison in the United States for processing and analyzing images. Continue this for the subsequent lanes (pressing Crtl and 2 every time).įor the last lane, repeat the procedure but press Ctrl and 3 to set the last lane. ImageJ is a program for personal computer, which is available for Linux, Windows and Mac. Press Ctrl and 1 to set first lane (Command and 1 on the Mac).Ĭlick the centre of the square and drag it across to the next lane. Use the square selection tool to highlight the first lane. Convert the image to 8-bit using ImageJ function (Image→Type→8-bit).
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